Qiaamp dna mini kit and qiaamp dna blood mini kit handbook. Qiagen qiaquick gel extraction kit 28704 and 28706. This protocol is designed to extract and purify dna of 70 bp. Up to 400 mg agarose can be processed per spin column. For gel extraction or cleanup of dna 70 bp 10 kb from enzymatic reactions. Qiaquick gel extraction kit protocol using a microcentrifuge chen. Dna ladder was run on a 2% agarose gel and 4 different fragment sizes 200 bp, 500 bp, 1 kb, and 5 kb were recovered using omega bioteks e. Qiavac vacuum manifolds 30 handling guidelines for qiavac 24 31 qiavac 6 32 ordering information 33 qiagen companies and distributors 35 qiaquick spin handbook 032001 3. For columns we recommend 4 c storage for periods greater than 1 year. Qiaquick gel extraction kit b 0 4 p b 0 4 fragments removed fragments recovered figure 1. Kit is a universal kit for dna extraction and purification from a wide variety of cell types, including blood, cells, tissues and toughtolyse samples including bacteria and yeast. For more information, please refer to the qiaquick spin handbook, march 2008. Automated extraction of gdna from buffy coat samples. Solutions that contain ethanol, isopropanol or mops should be sterilized by filtration only.
Qiaquick gel extraction kit 40 b 40 bp fragments removed fragments recovered recoveries of dna fragments in the size range between removed and recovered are not defined. Qiagen qiaquick gel extraction kit, 50 rxns, 30 to 50l elution volume, 10g binding capacity, dna sample, tube format, silica technology, manual processing, 70 bp to 10 kb fragment, fast and convenient procedure, for gel extractioncleanup of up to 10g dna 70. Qiagen plasmid purification handbook 082003 9 product use limitations qiagen, qiafilter, and endofree plasmid kits are developed, designed and sold for. Qiaquick nucleotide removal kit protocol 21 qiaquick gel extraction kit protocols 23 using a microcentrifuge 23 using a vacuum manifold 25 troubleshooting guide 28 appendix. The purelink quick gel extraction and pcr purification combo kit is designed to purify dna fragments from agarose gels.
Qiagen qiaquick gel extraction kit, 250 rxns, 30 to 50l elution volume, 10g binding capacity, dna sample, tube format, silica technology, manual processing, 70 bp to 10 kb fragment, fast and convenient procedure, for gel extraction cleanup of up to. The most versatile benchtop automation in the lab for consistent extraction and purification of dna, rna, protein, and cells. They are not to be used for human diagnostic or drug purposes or to be administered to humans unless expressly cleared for that purpose by the food and drug administration in the usa. Rapid regeneration and reuse of silica columns from pcr. If you get double bands consistently, do a gel extraction see below. Product information thermo scientific genejet gel extraction kit. Marker qiagen kit brain liver heart purelink kit qiagen kit purelink kit qiagen kit purelink kit marker figure 5. The minelute pcr purification kit and minelute gel extraction kit can be fully automated on the qiacube. L of qg buffer were added to 1 volume 100 mg gel slice of gel containing excised dna fragment. The innovative qiacube uses advanced technology to process qiagen spin columns, enabling seamless integration of automated, lowthroughput sample prep into your laboratory workflow. Qiaquick gel extraction kit 50 from qiagen sample to.
Recoveries of dna fragments in the size range between removed and recovered are not defined. Load more dna on the gel and take care that you excise the gel. Qiagen qiaquick gel extraction kit, 50 rxns, 30 to 50l elution volume, 10g binding capacity, dna sample, tube format, silica technology, manual processing, 70 bp to 10 kb fragment, fast and convenient procedure, for gel extraction. Minelute gel extraction kit 50 from qiagen selectscience. Any precipitate that forms in the buffers during storage can be redissolved by incubating briefly at 37 c, then cooling to room temperature before use. Dnafragments yes yes yes yes oligonucleotides no yes no no ligation yes yes yes yes nicktranslation yes yes yes yes pcr yes. Electrophoretic analysis of recovered dna from an agarose gel compared to the control. As assessed by agarose gel, the dna extracted from each sample had a molecular weight equal to or greater than 23 kb figure 2.
Lyophilized qiagen protease can be stored at room temperature 1525c for up to. Qiaquick pcr purification, qiaquick nucleotide removal, and qiaquick gel extraction. Download download qiagen 28704 manual read online read online qiagen 28704 manual qiaquick pcr purification kit protocol gel extraction principle qiagen buffer qg qiagen gel extraction low yield qiaex ii gel extraction kit qiagen gel extraction troubleshooting gel extraction protocol without kit qiagen kit 28704 protocol this protocol is designed to extract and purify dna of 70 bp to 10. Purelink quick gel extraction pcr purification combo kit offers the ability to perform both a gel extraction or a pcr purification a single kit. The kit utilizes a proprietary silicabased membrane technology in the form of. We use qiagen gel extraction kit and we have not faced such problem with concentration of dna. Monarch dna gel extraction kit reproducibly recovers dna over a broad range of molecular weights. Kit for total dna isolation from agarose gels agilent. Centrifugation tubes or vessels with suitable capacity for the volumes specified in the appropriate protocol. For departmental related matters, please visit the department of molecular, cell, and developmental biology website.
Disposable columns from qiagen pcr purification and gel extraction kits can be repeatedly regenerated and reused a minimum of five times. Thermo scientific genejet gel extraction kit thermo fisher. Dna fragments in a size range of 70 bp to 10 kb can be efficiently recovered. All samples were from 30 mg tissue input and showed a high level of rna integrity. The qiaquick gel extraction kit provides spin columns, buffers, and collection tubes for silicamembranebased purification of dna fragments from gels up to 400 mg slices or enzymatic reactions. The protocol describes the preliminary harvesting of bacteria and incubation with lysozyme to lyse their cell walls before dna purification. Why is the concentration of dna very low after a gel extraction. Rneasy minihandbook rneasy mini kit for purification of total rna from animal cells, animal tissues, bacteria, and yeast, and for rna cleanup rneasy protect mini kit for immediate stabilization of rna in harvested animal tissues and subsequent total rna purification rneasy plant mini kit for purification of total rna from plants and. Purelink quick gel extraction and pcr purification combo kit. The thermo scientific genejet gel extraction kit should be stored at room temperature 1525 c.
Using a microcentrifuge or vacuum manifold, dna ranging from 70 bp to 10 kb is purified from 124 samples. The kingfisher system technology provides an efficient workflow solution. For departmental related matters, please visit the department of molecular, cell, and developmental biology website phone. Automated purification and extraction thermo fisher. Quality control the quality of the presto 96 well gel extraction kit is tested on a lottolot basis by purifying dna fragments of various sizes from agarose gel. Qiaex ii agarose gel extraction protocol this protocol is designed for the extraction of 40bp to 50kb dna fragments from 0. Using the small gel rig, pour a 1% low melting point agarose gel 0.
For purification of dna fragments 40 bp to 50 kb from gels and solutions. Dna ranging from 70 bp to 10 kb is purified using a simple and fast bindwashelute procedure and an elution volume of 3050. If you get very faint bands, clean as usual using ampure, then concentrate product see below. Qiaquick gel extraction kit 250 from qiagen selectscience. The qiaquick pcr purification kit and qiaquick gel extraction kit can be fully automated on the qiacube. The gel solubilization solution dissolves an agarose gel slice from gels run in. I have one from qiagen but i only recover about 50 % of my dna. Qiagen qiaex ii gel extraction kit, 150 extractions, 20l elution volume, 5g10l binding capacity, dna sample, tube format, silica technology, manual processing, 40 bp to 50 kb fragment, ideal for restriction digestion, labeling, ligation, pcr, for purification of dna fragments.
Qiavac vacuum manifolds 30 handling guidelines for qiavac 6 31 qiavac 24 32 ordering information 33 qiagen companies and distributors 35 qiaquick spin handbook 072002 3. Qiagen genomictips are gravityflow, anionexchange tips that enable purification of dna of up to 150 kb from a wide range of sample types. The genejet gel extraction kit is designed for rapid and efficient purification of dna fragments from standard or lowmelting point agarose gels run in either tae or tbe buffer. Qiagen s qiaquick gel extraction kit is highly effective for extracting high purity, doublestranded dna from either pcr reactions or agarose gels following gel electrophoresis. For cleanup of other enzymatic reactions, follow the protocol as described for pcr samples or use the minelute reaction cleanup kit. Poor yield with gel extraction kit for about the umpteenth time now, i have gotten poor yield isolating my cut fragment from a 1% agarose gel. A mixture of 7 dna fragments ranging from 10 kb down to 0. Dna fragment bindingsize range automated dna cleanup the qiaquick pcr purification kit and qiaquick gel extraction kit can be fully automated on the qiacube. Minelute handbook 062004 11 adsorption to minelute membrane salt and ph dependence the minelute silicagel membrane is uniquely adapted to isolate dna from both aqueous solutions and agarose gels, and up to 5 g dna can bind to each minelute column.
Monarch nucleic acid purification kits are optimized for maximum performance and minimal environmental impact. The axyprep dna gel extraction kit employs optimized reagents in combination with a convenient miniprep column to purify dna fragments from either tae or tbe agarose gels regular and lowmelt. For gel extractioncleanup of up to 10 g dna 70 bp to 10 kb from enzymatic reactions. Qiaquick gel extraction kit qiagen, 28704 or qiaquick pcr purification kit qiagen, 28104, see below. Download download qiagen 28704 manual read online read online qiagen 28704 manual qiaquick pcr purification kit protocol gel extraction principle qiagen buffer qg qiagen gel extraction low yield qiaex ii gel extraction kit qiagen gel extraction troubleshooting gel extraction protocol without kit qiagen kit 28704 protocol this protocol is designed to extract and purify. Dna fragments of interest are extracted by solubilizing slices of an agarose gel. Dna adsorbs to the dneasy membrane in the presence of high concentrations. This protocol is designed for purification of total dna from grampositive bacteria. The binding buffers in minelute spin kits provide the correct salt concentration. Visual inspection of rna integrity by agarose gel electrophoresis. Genelute gel extraction kit 70 purifications sigmaaldrich. Qiagen research kits compatible with the qiavac 24 plus application kit accessories plasmid minipreps qiaprep spin miniprep kits dna cleanup qiaquick pcr purification kits qiaquick gel extraction kits qiaquick nucleotide removal kits minelute pcr purification kits. For uptodate licensing information and productspecific disclaimers, see the respective qiagen kit handbook or user.
The zymoclean gel dna recovery kits are gel dna extraction kits that provide rapid purification of highquality dna from taetbebuffered agarose gels. Automated extraction of gdna from buffy coat samples preserved. Automated dna cleanup the qiaquick pcr purification kit and qiaquick gel extraction kit can be fully automated on the. All pcr products were excised from the gel and purified using qiaquick gel extraction kit qiagen, germany. The kit was tested in the extraction of dna fragments from an agarose gel according to the protocol described in the manual. The qiaquick gel extraction kit provides spin columns, buffers, and collection tubes for silicamembranebased. Isolate a suitable piece of tissue and place in a uvcrosslinked 1. Gel extraction kit and a comparable kit from company q following manufacturers recommended protocols. Qiaquick gel extraction kit protocol using a microcentrifuge. If the purified dna is to be analyzed on a gel, add 1 volume of loading dye to. This protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or low.
I put in 1ug and i am getting back about 10nul but, my 260280s are jumping all over the place, as is my concentrations. Qiaquick pcr purification kit protocol using a microcentrifuge this protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8. Forceps are to be sterilized in fine science tools heat block at. Qiaquick gel extraction kit the qiaquick gel extraction kit cat. They can be stored for at least 2 years without showing any reduction in performance, capacity, or quality of separation. These gel dna extraction kits feature zymospin technology to yield highquality, purified dna in just minutes. Qiagen plasmid purification handbook harvard university. For gel extraction cleanup of up to 10 g dna 70 bp to 10 kb from enzymatic reactions. Banerjee lab ucla molecular, cell and developmental biology. Qiaex ii handbook qiaex ii agarose gel extraction protocol. Qiagen qiaquick gel extraction kit, 50 rxns, 30 to 50l elution volume, 10g binding capacity, dna sample, tube format, silica technology, manual processing, 70 bp to 10 kb fragment, fast and convenient procedure, for gel extraction cleanup of up to 10g dna 70. Qiagen qiaquick gel extraction kit, 250 rxns, 30 to 50l elution volume, 10g binding capacity, dna sample, tube format, silica technology, manual processing, 70 bp to 10 kb fragment, fast and convenient procedure, for gel extractioncleanup of up to 10. Qiaquick gel extraction and pcr purification kit from qiagen. Qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or lowmelt agarose gels in tae or tbe buffer.
Add ethanol 96100% to buffer pe before use see bottle label for volume. Each fragment was manually excised from the agarose gel and processed using the monarch dna gel extraction kit. The qiaquick gel extraction kit enables removal of nucleotides, enzymes, salts, agarose, ethidium bromide, and other impurities from samples, ensuring up to 80% recovery of dna see figure high recoveries from gels. Qiagen plasmid purification handbook 082003 5 kit contents. Qiaquick pcr nucleotide qiaquick gel qiaquick gel purification kit removal kit extraction kit extraction kit alkalinephosphatase yes yes yes yes cdnasynthesis yes no no yes dnase, yes yes yes yes nucleasedigestion kinase. For maximum convenience and value, columns and buffers are also available separately. Qiaex ii gel extraction kit 150 from qiagen selectscience. It eliminates resin manipulation, toxic phenol chloroform extractions, and ethanol precipitations. This kit can also be used for dna cleanup from enzymatic reactions see page 8. Agarose gel analysis of the purification procedure 60.